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| Ocarino,N.M.; Bozzi,A.; Pereira,R.D.O.; Breyner,N.M.; Silva,V.L.; Castanheira,P.; Goes,A.M.; Serakides,R.. |
| 4&', 6-diamidino-2-phenylindole dihydrochloride (DAPI) is a DNA dye widely used to mark and trace stem cells in therapy. We here studied the effect of DAPI staining on the behavior of mesenchymal stem cells cultured in either a control, non-osteogenic medium or in an osteogenic differentiation medium. In the control medium, the number of stem cells/field, as well as the number of fluorescent cells/field increased up to the sixth day in both control and DAPI-treated cultures. Afterwards, both the number of fluorescent cells and their fluorescence intensity decreased. Control cells were fusiform and with some long extensions that apparently linked them to neighboring cells, while DAPI-treated cells were mostly round cells with fine and short extensions.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bone marrow; Cell cultures; Rat; Osteogenic differentiation. |
| Ano: 2008 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452008000200003 |
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| Duarte,D.P.F.; Silva,V.L.; Jaguaribe,A.M.; Gilmore,D.P.; Da Costa,C.P.. |
| Blood pressure (BP) profiles were monitored in nine free-ranging sloths (Bradypus variegatus) by coupling one common carotid artery to a BP telemetry transmitter. Animals moved freely in an isolated and temperature-controlled room (24ºC) with 12/12-h artificial light-dark cycles and behaviors were observed during resting, eating and moving. Systolic (SBP) and diastolic (DBP) blood pressures were sampled for 1 min every 15 min for 24 h. BP rhythm over 24 h was analyzed by the cosinor method and the mesor, amplitude, acrophase and percent rhythm were calculated. A total of 764 measurements were made in the light cycle and 721 in the dark cycle. Twenty-four-hour values (mean ± SD) were obtained for SBP (121 ± 22 mmHg), DBP (86 ± 17 mmHg), mean BP (MBP, 98 ±... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Sloths; Bradypus; Circadian rhythm; Blood pressure. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000200016 |
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| Lafetá,B.N.; Santos,S.; Silva,V.L.; Carvalho,M.A.R.; Diniz,C.G.; Silva,N.. |
| Estudou-se o perfil das proteínas da membrana externa (PME) da Leptospira interrogans sorovariedade Hardjoprajitno por meio da eletroforese bidimensional. Foram utilizadas técnicas de extração das PME com Triton x114 e precipitação com acetona. Os géis foram corados com nitrato de prata e as imagens analisadas para determinação da massa molecular das proteínas detectadas. Foram visualizadas 35 bandas protéicas, sendo que cinco delas se destacaram por estarem em maior quantidade: 22,54KDa (LipL22), 30/26KDa (LipL32), 34,41KDa (PME34), 42,75KDa (LipL41) e 58,59KDa (LipL63). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira interrogans Hardjoprajitno; Proteínas de membrana; Eletroforese bidimensional. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600002 |
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| Coutinho,P.S.; Ocarino,N.M.; Ferreira,M.B.; Resende,R.; Silva,V.L.; Guedes,R.M.C.; Serakides,R.. |
| Nine cases of familial osteopetrosis were studied in Agouti paca rodents maintained in captivity. Animals were distributed in three groups depending on the severity of their skeletal lesions. Based upon clinical, radiological, and microscopic findings, it was concluded that one animal had level I lesions, three animals had level II lesions, and five animals had level III osteopetrosis and osteonecrosis. Throughout the entire axial and appendicular skeleton, there was an increased amount of both trabecular and cortical bone tissue. All analyzed bones showed thickened cortex and reduced medullary canals. Bone trabeculae were thick and confluent. Cortex showed a narrowing of Haversian canals. Numerous cementing lines resulted in typical mosaic patterns.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Agouti paca; Familial disease; Osteonecrosis; Osteopetrosis; Skeleton. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000600022 |
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